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Fig. 1 | BMC Immunology

Fig. 1

From: Recombinant ArtinM activates mast cells

Fig. 1

Analysis of rArtinM and jArtinM and analytical ultracentrifugation assay. a Lane 1: undenatured rArtinM. Lane 2: rArtinM after thermal dissociation. Lane 3: undenatured jArtinM. Lane 4: jArtinM after thermal dissociation. 3 μg of protein were loaded to each lane. 12.5 % SDS-PAGE stained with Coomassie blue G-250. b Size distribution obtained from the sedimentation velocity profiles of rArtinM at 20 °C. Fit and residuals after fitting to a c(S) were calculated in SEDFIT. Plot of the distribution of sedimentation coefficients (C(s)) versus (S), where S is plotted in Svedberg units. The shape of the major peak shows oligomeric structures of rArtinM. By non-linear fitting, the average molecular weight (Mw) for rArtinM was determined to be 50 kDa (displayed inside the plot)

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