Fig. 3

High doses of rArtinM are required for mast cell mediator release. a In the absence of IgE only 20 and 40 μg/ml of rArtinM induced β-hexosaminidase release, and in the presence of IgE all doses induced β-hexosaminidase release. b In the absence of IgE only 40 μg/ml of rArtinM stimulated release of TNF-α, whereas in IgE sensitized cells 10 and 40 μg/ml of rArtinM induced TNF-α release. c The release of LTC4 was seen only in IgE sensitized cells. d rArtinM stimulation of IL-4 release did not require IgE. Data are expressed as mean ± SEM and are representative of three separate experiments. *p < 0.05 or ** p < 0.01 or *** p < 0.001 between samples and the controls