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Fig. 1 | BMC Immunology

Fig. 1

From: Avidity characterization of genetically engineered T-cells with novel and established approaches

Fig. 1

Lentiviral vector for expression of recombinant TCRs. A self-inactivating lentiviral vector was constructed with recombinant TCR α and β chains with human variable (V), diversity (D) and joining (J) gene segments and mouse constant (C) domains. The TCR α and β chains are separated by a self-cleaving T2A peptide and the SFFV promoter controls TCR expression. Lentivirus particles were produced in 293 T-cells and concentrated by ultracentrifugation before being used to transduce human T-cells. Abbreviations: TCR = T-cell receptor; LTR = long terminal repeat; SIN = self inactivating (deletion of the direct repeat enhancer); SSFV = spleen-focus forming virus; V = variable, D = diversity and J = joining gene segment; T2A = 2A peptide derived from Thosea asigna virus

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