Fig. 5
From: Avidity characterization of genetically engineered T-cells with novel and established approaches
Target cell death in response to pp65-TCR and TARP-TCR-engineered T-cells in real time. Mel526 target cells were plated in E-Plate View and continuously monitored for growth using the xCELLigence DP Instrument for approximately 24 h. a The mel526 target cells in the E-Plate View were then pulsed with pp65495–503 peptide at 10 μM (Slateblue), 100 nM (Coral) or 1nM (Cyan), or TARP4–13 peptide at 10 μM (Magenta), 100 nM (Green) or 1 nM (Blue), or irrelevant VMAT-131–39 peptide as a control (Saddlebrown) for 2 h. The pp65-TCR or TARP-TCR-engineered T-cells were then added at effector to target cell ratio of 5:1. The Cell Index measured was normalized to the time point of T-cell addition. Arrows indicate peptide and T-cell addition. b The mel526 target cells in the E-Plate View were pulsed with pp65495–503 and TARP4–13 peptide at 10 μM for 2 h. pp65-TCR T-cells were then added in effector to target ratios 1:1 (Cyan), 5:1 (Coral) or 10:1 (Slateblue) or TARP-TCR T-cells were added at ratios 1:1 (Green), 5:1 (Blue) or 10:1 (Magenta), or no T-cells were added as a control (Saddlebrown). Arrows indicate peptide and T-cell addition. c Growth curves between 26 and 30 h of measurement, i.e., 0 to 4 h post addition of T-cells, are magnified from A (Left) and B (right)