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Fig. 5 | BMC Immunology

Fig. 5

From: Repeated cycles of 5-fluorouracil chemotherapy impaired anti-tumor functions of cytotoxic T cells in a CT26 tumor-bearing mouse model

Fig. 5

Cytotoxicity and cytokine production after 5-FU C1 and C3 treatment. a Cytotoxicity against CT26 using CFSE-PI staining-based flow cytometry. Spleen cells from treatment and control groups as effectors were incubated with CFSE-stained CT26 cells (Fig. 5a) at an effector:target (E:T) ratio of 25:1. CFSE and PI positive cells represent killed target cells, and the cytotoxicity was calculated. b Cytotoxicity against 4T1 cells, and c cytotoxicity against YAC-1 cells at the E:T ratio of 25:1. d IFN-γ production by spleen cells in the MLTC assay. The supernatant of MLTC was collected on day 3, and the IFN-γ concentration was analyzed by ELISA. e Serum TGF-β was quantified by ELISA. Serum was collected from C1, C3 and control groups (n = 3) on day 7 after the last 5-FU injection. Student’s t-test was used to analyze the significance between groups. The experiments were replicated at least twice with similar results

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