Fig. 3
From: Active immunization with human interleukin-15 induces neutralizing antibodies in non-human primates
Abs response in macaques immunized with mhIL-15 corresponding to 15 days after the third immunization. a ELISA for Abs titers against IL-15. The plate was coated with 1 μg/ml mhIL-15 and the serum from each animal was evaluated in twofold serial dilutions (starting dilution 1:1000). All animals developed an Abs response, except pre-immune and placebo macaques. The line represents the mean values of Abs titers calculated from duplicate samples of individual monkeys (n = 3) corresponding to each experimental group. b ELISA for recognition of native IL-15 by Abs from sera of immunized macaques. The plate was coated with 1 μg/ml native human IL-15 and the pool of sera from each group was evaluated in fixed dilution, 1:4000. c Serum neutralization titers of macaques calculated from the data obtained in the CTLL-2 cell proliferation assay. The line represents the mean values of neutralization titers (expressed as 1/ID50) calculated from duplicate samples of individual animals (n = 3). The ID50 was determined by inhibiting of human IL-15-induced proliferation of CTLL-2 cells