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Fig. 1 | BMC Immunology

Fig. 1

From: Silencing of CD47 and SIRPα by Polypurine reverse Hoogsteen hairpins to promote MCF-7 breast cancer cells death by PMA-differentiated THP-1 cells

Fig. 1

Schematic representation of co-culture experiments. MCF-7 and THP-1 cells were plated in separate dishes and THP-1 cells were immediately transfected with PPRHs against SIRPα. After 24 h, transfected THP-1 cells were added to MCF-7 cells and differentiated to macrophages with PMA for 3 days. After that period of time, the medium was replaced with fresh F12 medium and PPRHs against CD47 were transfected. Cell viability (MTT) assay was assessed 5 days after the last transfection

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