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Fig. 1 | BMC Immunology

Fig. 1

From: Intranasal immunization with recombinant Toxoplasma gondii actin depolymerizing factor confers protective efficacy against toxoplasmosis in mice

Fig. 1

PCR detection of TgADF and SDS-PAGE and western blotting of rTgADF. a ORF of TgADF was amplified using PCR and examined with 1 % agarose gel electrophoresis. The length of the ORF was 357 bp. b TgADF was expressed in E. coli, purified using Ni-NTA agarose, detected with 12 % SDS-PAGE, and stained with Coomassie brilliant blue. Lane 1: Purified rTgADF displayed an apparent molecular mass of 20 kDa. c Western blotting showed that purified rTgADF (lane 2) reacted with anti-His antibody or rabbit anti-T. gondii serum, whereas E. coli containing the empty plasmid induced by IPTG did not show any band (lane 1)

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BMC Immunology

ISSN: 1471-2172

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