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Fig. 3 | BMC Immunology

Fig. 3

From: A new high-throughput sequencing method for determining diversity and similarity of T cell receptor (TCR) α and β repertoires and identifying potential new invariant TCR α chains

Fig. 3

Heatmap representation of gene recombination of TRAV with TRAJ in pooled read data from 20 healthy individuals. The numbers of TCR sequence reads bearing respective gene recombination of TRAV and TRAJ were counted. The occurrence tendency of recombination is visualized by heat map presentation of the number of each recombination. Color in each pixel indicates the number of each recombination. For TRAV, 8 pseudogenes (TRAV8-5, TRAV11, TRAV15, TRAV28, TRAV31, TRAV32, TRAV33, and TRAV37), 1 ORF (TRAV8-7), and poorly expressed genes (TRAV7, TRAV9-1, TRAV18 and TRAV36) were excluded. For TRAJ, 3 pseudogenes (TRAJ51, TRAJ55, and TRAJ60), 6 ORFs (TRAJ1, TRAJ2, TRAJ19, TRAJ25, TRAJ59, and TRAJ61), and poorly expressed genes (TRAJ14 and TRAJ46) were excluded. Two ORFs (TRAJ35 and TRAJ48) found to be expressed were included. A heat map representation of the 2050 recombination events (41 TRAV × 50 TRAJ) is shown

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BMC Immunology

ISSN: 1471-2172

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