A new high-throughput sequencing method for determining diversity and similarity of T cell receptor (TCR) α and β repertoires and identifying potential new invariant TCR α chains

Table 1 Primers used in this study

Primer	Sequence	MID Tag
BSL-18E	AAAGCGGCCGCATGCTTTTTTTTTTTTTTTTTTVN
P20EA	TAATACGACTCCGAATTCCC
P10EA	GGGAATTCGG
CA1	TGTTGAAGGCGTTTGCACATGCA
CA2	GTGCATAGACCTCATGTCTAGCA
CB1	GAACTGGACTTGACAGCGGAACT
CB2	AGGCAGTATCTGGAGTCATTGAG
HuVaF-01 ~ 10	CCATCTCATCCCTGCGTGTCTCCGAC TCAG-{MID}-ATAGGCAGACAGACTTGTCACTG	MID1 ~ MID11
HuVbF-01 ~ 10	CCATCTCATCCCTGCGTGTCTCCGAC TCAG-{MID}-ACACCAGTGTGGCCTTTTGGGTG	MID15 ~ MID24
B-P20EA	*CCTATCCCCTGTGTGCCTTGGCAGTC*TAATACGACTCCGAATTCCC

V: A/C/G, N: A/C/G/T, Adaptor A and B sequences were typed in bold and bold italic, respectively. A key sequence (TCAG) was underlined. The following MID Tag sequences were used for identification of sample source. MID1: ACGAGTGCGT, MID2: ACGCTCGACA, MID3: AGACGCACTC, MID4: AGCACTGTAG, MID5: ATCAGACACG, MID6: ATATCGCGAG, MID7: CGTGTCTCTA, MID8: CTCGCGTGTC, MID10: TCTCTATGCG, MID11: TGATACGTCT, MID15: TACGACGTA, MID16: TCACGTACTA, MID17: CGTCTAGTAC, MID18: TCTACGTAGC, MID19: TGTACTACTC, MID20: ACGACTACAG, MID21: CGTAGACTAG, MID22: TACGAGTATG, MID23: TACTCTCGTG, MID24: TAGAGACGAG

ISSN: 1471-2172