Fig. 4

Vaccination with vaccine in a chitosan gel increases the frequency of OVA-specific CD8⁺ T cells post challenge with tumour. Mice received adoptive transfer of OT-I lymphocytes and were vaccinated with vaccine in a chitosan gel (Gel + OVA), the gel alone (Gel) or with vaccine pulsed-DC (DC + OVA) then challenged intracaecally with B16-OVA 30 days later. a, b Percentage of Vα2⁺Vβ5⁺ T cells of CD8⁺ T cells is shown in peripheral lymph nodes (a) and spleens (b) at day 21. c, d Percentage of CD122⁺CD44⁺ of Vα2⁺Vβ5⁺ CD8⁺ T cells is shown in peripheral lymph nodes (c) and spleens (d) at 21 days following challenge. Data are shown as mean ± standard deviation, n = 4–5, representative of two individual experiments. One-way ANOVA with Tukey post-hoc test was performed. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001