Fig. 5

Vaccination with Gel + OVA increases cytokine production in the spleen. Mice received adoptive transfer of OT-I lymphocytes and were not vaccinated or were vaccinated with vaccine in a chitosan gel (Gel + OVA), the gel alone (Gel) or with vaccine pulsed-DC (DC + OVA) then challenged intracaecally with B16-OVA 30 days later. Percentage (a, c, e) and number (b, d, f) of IFN-γ⁺ CD8⁺ T cells (a, b), IFN-γ ⁺ CD4⁺ T cells (c, d), IFN-γ ⁺ Vα2Vβ5⁺ CD8⁺ T cells (e, f). All data shown are from spleen cells restimulated with PMA/ionomycin for 4 h, 21 days following challenge. Data are shown as mean ± standard deviation, n = 4–5, representative of two individual experiments. One-way ANOVA with Tukey post-hoc test was performed. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001