Fig. 4

Effect of HSdelip or HSdelip supplemented with VLDL, LDL or HDL on bacteria-induced TLR2 activity. HEK-TLR2-TLR6 cells were incubated with S. aureus ( a ), E. coli ( b ), L. salivarius ( c ), S. typhimurium ( d ) or B. breve ( e ) at a ratio of 10:1 (black bars) or 25:1 (grey bars) (bact:cell) in the presence of HS or HSdelip supplemented with the different lipoprotein fractions. HDL, LDL or VLDL significantly inhibited bacterial induced TLR2 activation. Each value represents mean ± SD of triplicates. The experiment shown is representative of three separate experiments. *:p < 0.05 **:P < 0.01 ***:P < 0.001 determined by comparison with HSdelip 10:1. ^$:p < 0.05 ^$$:P < 0.01 ^$$$:P < 0.001 determined by comparison with HSdelip 25:1