Fig. 5

Effect of HS, HSdelip or HSdelip supplemented with VLDL, LDL or HDL on the ability of bacterial-ligands or bacteria to induce TLR2 or TLR4 activity. Bacteria or bacterial ligands were preincubated for the indicated times with either HS, HSdelip (HSd) or HSdelip supplemented with the separate lipoproteins before the addition of TLR-transfected cells. TLR2 activity in response to ligation by B. breve (a) and L. salivarius (b) was attenuated by HS or HSdelip supplemented with VLDL, LDL or HDL. No effect between the different preincubation times could be observed. TLR2 activity in response to ligation with E. coli (c), S. thyphimurium (d) or FSL-1 (e) was attenuated by HS or HSdelip supplemented with VLDL, LDL or HDL. This effect increased with prolonged preincubation. TLR4 activity in response to ligation with E. coli (f) or LPS (g) was attenuated by HS or HSdelip supplemented with VLDL, LDL or HDL. This effect increased with prolonged preincubation. Each value represents mean ± SD of triplicates. The experiment shown is representative of three separate experiments. *: p < 0.05 **:P < 0.01 ***:P < 0.001 determined by comparison with HSdelip