The UT family of MHC class I loci unique to non-eutherian mammals has limited polymorphism and tissue specific patterns of expression in the opossum

Krasnec, Katina V.; Papenfuss, Anthony T.; Miller, Robert D.

doi:10.1186/s12865-016-0181-9

Table 1 List of gene specific primers sets used for PCR and cDNA for RACE

From: The UT family of MHC class I loci unique to non-eutherian mammals has limited polymorphism and tissue specific patterns of expression in the opossum

Gene	Exon 2 F	Exon 2 R	Intron 2 F	Intron 2 R	Exon 3 F	Exon 3 R	Length	Overlap
ModoUT2	TGCTCTCCAGCTCTCCACA					ATCTTCAGAAATGTTGGGCACT	689 bp
ModoUT3	ACCACAGCCATATAGGTCAGTTC					TCAAGTCAACACAATAGAGCTTCA	824 bp 812 bp,
ModoUT4	CCTTCCTATAATCTAGTCCACCACA		CAATCATGGGGGTTAGAAACAT	TCCCCAAATTCTTAGTGCAGTT		TCCAACAATTTTCTTCATCAAGTC	742 bp	207 bp
ModoUT5	CCTATAATTCAGTCCACCACAGG					CTCACCATTATCCCTTATGCTTG	855 bp 837 bp,
ModoUT6	CCTTCCTATAATTCAGTCCACCA		GTCCCATGCTCTTTTTCAGTTC	TCTCATGTTATGCCATGTGTCC		TTCCTTAAGTTTGAATACCCAATG	779 bp 753 bp,	244 bp
ModoUT7	CACACTATAACTTCATGTAGTGTTTCC		GCTGCTTGCAGGAGTAAAAGAT	GACTTTCAGAGTTTGCCAAAGG		CCTATAATTCAGTCCACCACAGG	682 bp 816 bp,	150 bp
ModoUT8	GTTCTGCAGCTCACCACAAAC		CTCATCCACCAGTGTGTTGTTC	GGGTAGGAGCTTCATGTCAGTC		CTTGCATGTAGTGCTTCCTCTG	738 bp 286 bp,	96 bp
ModoUT9	CCCATATATGACCTGCTTTCCT	GTGGAGGTGGAAATAAGTGAGG			TTAGCCTGGCCATAATACTTCC	GGGAAATATGACTTGTACCTCCTG	450 bp 346 bp,
ModoUT10	CCCCTACCTTTCTGTCATTCAG	GGACAAGCGCTTTTTGAGTATG			TTCATCTCCAATTCTGATTCCA	GATTGGTTAGAGCCTGTGCTTT	354 bp 833 bp,
ModoUT15	GCTTTCACTCCTGTCTCTCAGC		ACCTCGAAGGTCATCTAGTCCA	TGTCCTTTGAAACCTAGGCAAT		TCATGCTTGAATGCTGTAGAAC	876 bp 229 bp,	138 bp
ModoUT17	CTTTTCTGTCCTTTAGCCCATC	CCCCTCTCTTATCATTCTTTACC			CCAGAGAACCATACAGTCCAGAT	TGTTTGACTGTAGGACTTTCCTC	210 bp
	5’ RACE R	5’ RACE R nested	3’ RACE F	3’RACE F nested	overlap
ModoUT4	TTCATGTTCTCCCAGTGCCCAACC		GAAACCACACAGCCCAGCTCCAG		89 bp
ModoUT5	TGTGCCAACTCCAGTCAACTGTCCC		GGGACAGTTGACTGGAGTTGGCACA		0 bp
ModoUT6	AGACTGGTCCAGAGGGGACTCTC		CTGGAGAGTCCCCTCTGGACCAGTCT		0 bp 363 bp,
ModoUT8	GTGGTCCACAGGTTACTTGTCA	TGGATTTCATTTCCTAGCTGGT	GGAAGCTCCCTCTTGGAATACT	TCATAGAGGACACTTGGCAGAA	119 bp

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ISSN: 1471-2172

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