TY - JOUR AU - Surenaud, Mathieu AU - Manier, Céline AU - Richert, Laura AU - Thiébaut, Rodolphe AU - Levy, Yves AU - Hue, Sophie AU - Lacabaratz, Christine PY - 2016 DA - 2016/11/11 TI - Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells JO - BMC Immunology SP - 44 VL - 17 IS - 1 AB - The Luminex bead-based multiplex assay is useful for quantifying immune mediators such as cytokines and chemokines. Cross-comparisons of reagents for this technique from different suppliers have already been performed using serum or plasma but rarely with supernatants collected from antigen-stimulated peripheral blood mononuclear cells (PBMC). Here, we first describe an optimization protocol for cell culture including quantity of cells and culture duration to obtain reproducible cytokine and chemokine quantifications. Then, we compared three different Luminex kit suppliers. SN - 1471-2172 UR - https://doi.org/10.1186/s12865-016-0182-8 DO - 10.1186/s12865-016-0182-8 ID - Surenaud2016 ER -