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Fig. 2 | BMC Immunology

Fig. 2

From: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

Fig. 2

Optimization of cell concentration. Milliplex kits from Millipore (see Material and Methods) were used to perform cell concentration optimization experiments using 0.5 or 1.0 million Non-Stimulated (NS) or PPD-Stimulated PBMC from two healthy donors cultured in triplicate for 2 or 5 days. Results obtained with samples from donor 1 (IL-2 and IL-17) and donor 2 (IFNγ and IL-5) are shown at the optimal time point defined for each cytokine. Mean cytokine concentrations are represented by black lines

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