Table 1 Description of Luminex kits

BIIR + Biolegend

-

Polystyrene

4

IFNγ, IL-5, IL-17 + Perforin

Reproducibility

Millipore

Milliplex MAP Human Cytokine (#SPR137)

Magnetic

11

IL-17, IL-21, IP-10, IFNγ, IL-10, IL-13, IL-1β, IL-2, IL-5, IL-6, TNFα

Kinetics of cytokine production + Quantity of stimulated PBMC

Millipore

Milliplex MAP Human Cytokine (#SPR171)

Polystyrene

10

IL-17, IL-21, IFNγ, IL-10, IL-13, IL-1β, IL-2, IL-5, IL-6, TNFα

Kinetics of cytokine production + Quantity of stimulated PBMC

Millipore

Milliplex MAP Human Th17

Magnetic

10

IL-17A, IL-22, IFNγ, IL-10, IL-13, IL-1β, IL-2, IL-5, IL-6, TNFα

Kinetics of cytokine production + Quantity of stimulated PBMC

Ozyme

Procarta Immunoassays kit (Affymetrix)

Magnetic

16

GM-CSF, IFNγ, IL-10, IL-12p40, IL-13, IL-17A, IL-1β, IL-2, IL-22, IL-5, IL-6, IP-10, MCP-1, MIP-1α, MIP-1β, TNFα

Comparison of commercial Luminex Kits

Millipore

Milliplex MAP Human Cytokine (#SPR158) + Milliplex MAP Human Cytokine/Chemokine

Magnetic

15 + 1

GM-CSF, IFNγ, IL-10, IL-13, IL-17A, IL-1β, IL-2, IL-22, IL-5, IL-6, IP-10, MCP-1, MIP-1α, MIP-1β, TNFα + IL-12p40

Comparison of commercial Luminex Kits

Bio-Rad

Bio-plex Pro assays + Bio-Plex Pro Human Th17 Cytokine assays

Magnetic

28 + 1

IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, Eotaxin, Basic FGF, G-CSF, GM-CSF, IFNγ, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-BB, RANTES, TNFα, VEGF, and IL-12p40 + IL-22

Comparison of commercial Luminex Kits

Millipore

Milliplex MAP Human Th17

Magnetic

15

GM-CSF, IFNγ, IL-10, IL-17 F, IL-13, IL-17A, IL-1β, IL-2, IL-22, IL-9, CCL20/MIP-3α, IL-5, IL-23, IL-27, and TNFα

Internal QC study