Fig. 1

ZA inhibits Treg cells proliferation and induces cell injury. a Expanded Treg cells were labeled with CFSE and cultured in Treg cell medium with or without 10 μM ZA. b Treg cell proliferation curves were measured based on the percentage of cells with decreased fluorescence as compared to non-proliferating cells (0.38% at day 1). Data represent the mean values ± SEM and results from three independent experiments are shown. Statistical significance (P < 0.01) is denoted by **. c The morphology of Treg cells was analyzed by microscopy in 100× oil immersion after ZA treatment for 24 h