Fig. 2From: Immune modulation of CD4+CD25+ regulatory T cells by zoledronic acidZA inhibits Treg cells chemotactic migration. Treg cells (5 × 10 4) were pretreated with 0, 50 or 100 μM ZA for 4 h, and placed in the upper chambers. Migration of Treg cells into the lower chambers containing DMEM with 2% FBS or CM from MDA-MB-231 cells after 2 h was analyzed. The chemotaxis index shown compares migration with the response of control cells to DMEM with 2% FBS. Values are means ± SEM of results from three independent experiments in duplicate. *P < 0.05, ** p < 0.01, ***p < 0.001Back to article page