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Table 1 Primer sequences used for quantitative RT-PCR for mRNA expression

From: Immune modulation of CD4+CD25+ regulatory T cells by zoledronic acid

Target gene Oligonucleotide sequences (5′-3′)
Forward Reverse
Foxp3 TGA CCA AGG CTT CAT CTG TG GAG GAA CTC TGG GAA TGT GC
CCR4 CTT CAG TCA CCT GGC TGT CA CTC AGC TGA ACC TGG CTA CC
TGF-β GGA AAC CCA CAA CGA AAT C GCT CTG ATG TGT TGA AGA AC
RANKL ACC AGC ATC AAA ATC CCA AG CCC CAA AGT ATG TTG CAT CC
CTLA-4 CTC AGC TGA ACC TGG CTA CC CCA CTT GCA GAC ACC ATT TG
PD-1 ATC AAA GAG AGC CTG CGG G GGT GGG CTG TGG GCA CT
GAPDH TGA ACG GGA AGC TCA CTG G TCC ACC ACC CTG TTG CTG TA