Table 1 Primer sequences used for quantitative RT-PCR for mRNA expression
From: Immune modulation of CD4+CD25+ regulatory T cells by zoledronic acid
Target gene | Oligonucleotide sequences (5′-3′) | |
|---|---|---|
Forward | Reverse | |
Foxp3 | TGA CCA AGG CTT CAT CTG TG | GAG GAA CTC TGG GAA TGT GC |
CCR4 | CTT CAG TCA CCT GGC TGT CA | CTC AGC TGA ACC TGG CTA CC |
TGF-β | GGA AAC CCA CAA CGA AAT C | GCT CTG ATG TGT TGA AGA AC |
RANKL | ACC AGC ATC AAA ATC CCA AG | CCC CAA AGT ATG TTG CAT CC |
CTLA-4 | CTC AGC TGA ACC TGG CTA CC | CCA CTT GCA GAC ACC ATT TG |
PD-1 | ATC AAA GAG AGC CTG CGG G | GGT GGG CTG TGG GCA CT |
GAPDH | TGA ACG GGA AGC TCA CTG G | TCC ACC ACC CTG TTG CTG TA |