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Table 2 Cross priming capability of L-DC compared with myeloid cells

From: Antigen presenting capacity of murine splenic myeloid cells

Expt

Condition

T cell: APC ratio giving 50% maximum proliferation of OT-I T cellsab

L-DC

Resi Mono

Infl mono

Neu

cDC

I

+ LPS

33

-

-

33

300

- LPS

33

-

-

<33

300

II

+ LPS

33

33

-

-

300

- LPS

33

<33

-

-

300

III

+ LPS

100

42

-

-

300

- LPS

100

42

-

-

141

IV

+ LPS

100

42

<33

-

300

- LPS

100

33

<33

-

300

V

- LPS

100

33

0

<33

-

VI

+ LPS

141

0

-

<33

-

- LPS

100

0

-

<33

-

  1. aFor preparation of APC, splenocytes were harvested from Act-mOVA mice and prepared by red blood cell lysis followed by T and B cell depletion. L-DC, cDC, resident monocytes (Resi mono), inflammatory monocytes (Infl mono), neutrophils (Neu) were sorted as described in Table 1
  2. b APC, with and without LPS (10ug/ml), were cocultured with 105 CFSE-labelled OT-I (TCR-tg) CD8+ T cells, sorted as PIThy1.2+Vα2+CD4 cells, at T cell:APC ratios of 33, 100, 300 and 900:1. After 72 h, CD8+ OT-I T cells were gated as PICD11bThy1.2+Vα2+ cells, and CFSE dilution assessed flow cytometrically to estimate % proliferating T cells. OT-I T cells alone served as a control (Con)