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Table 2 Cross priming capability of L-DC compared with myeloid cells

From: Antigen presenting capacity of murine splenic myeloid cells

Expt Condition T cell: APC ratio giving 50% maximum proliferation of OT-I T cellsab
L-DC Resi Mono Infl mono Neu cDC
I + LPS 33 - - 33 300
- LPS 33 - - <33 300
II + LPS 33 33 - - 300
- LPS 33 <33 - - 300
III + LPS 100 42 - - 300
- LPS 100 42 - - 141
IV + LPS 100 42 <33 - 300
- LPS 100 33 <33 - 300
V - LPS 100 33 0 <33 -
VI + LPS 141 0 - <33 -
- LPS 100 0 - <33 -
  1. aFor preparation of APC, splenocytes were harvested from Act-mOVA mice and prepared by red blood cell lysis followed by T and B cell depletion. L-DC, cDC, resident monocytes (Resi mono), inflammatory monocytes (Infl mono), neutrophils (Neu) were sorted as described in Table 1
  2. b APC, with and without LPS (10ug/ml), were cocultured with 105 CFSE-labelled OT-I (TCR-tg) CD8+ T cells, sorted as PIThy1.2+Vα2+CD4 cells, at T cell:APC ratios of 33, 100, 300 and 900:1. After 72 h, CD8+ OT-I T cells were gated as PICD11bThy1.2+Vα2+ cells, and CFSE dilution assessed flow cytometrically to estimate % proliferating T cells. OT-I T cells alone served as a control (Con)