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Table 2 Immune responses against Giardia and control antigens as measured by 3H-thymidine proliferation assay

From: Giardia-specific cellular immune responses in post-giardiasis chronic fatigue syndrome

 

Exposed

Unexposed

p-values

Stimulation agent

No PI-fatigue controls (n = 19)

PI-CFS & ICF (n = 19)

Fatigue other cause (n = 9)

Recovered from fatigue (n = 9)

Healthy controls (n = 10)

Exposed n = 56)vs unexposed (n = 10)

No PI-fatigue controls (n= 19) vs PI-CFS/ICF(n = 19)

Giardia ass A 10 μg/ml

12.8 (13.0)

15.3 (19.9)

20.3 (10.9)

15.1 (65.0)

3.5 (6.2)

0.001

ns*

Giardia ass B 10 μg/ml

12.2 (13.9)

13.3 (15.3)

23.3 (13.4)

15.3 (39.0)

3.5 (4.7)

<0.001

ns*

Tuberculin (PPD)10 μg/ml

26.3 (34.1)

34.0 (47.5)

54.9 (84.5)

42.5 (23.2)

27.7 (27.0)

ns

ns*

LP S Stypi 1 μg/ml

21.5 (18.6)

14.4 (13.2)

38.2 (34.3)

21.9 (19.5)

17.7 (10.5)

ns

ns*

C albicans 10 μg/ml

7.0 (9.1)

5.8 (11.9)

10.0 (32.0)

14.6 (6.0)

11.0 (9.4)

ns

ns*

aCD3aCD28(pos ctr)

70.6 (35.4)

51.4 (33.0)

51.8 (44.1)

51.8 (63.9)

60.1 (39.8)

ns

ns*

  1. *KruskalWallis test across all exposed groups was not significant (below p = 0.05). ns = not significant
  2. Proliferation responses are expressed as median stimulation indices (counts per minute(cpm) in stimulated triplicate cultures divided by cpm in unstimulated triplicate cultures) followed by standard deviation