Fig. 5From: Tumor necrosis factor alpha inhibitors have no effect on a human T-lymphotropic virus type-I (HTLV-I)-infected cell line from patients with HTLV-I-associated myelopathyAfter HCT-5 cells were stimulated with PBS or 10 μg g/ml of a TNFi for 48 h, they were treated with a mouse monoclonal anti-HTLV-I p19, p28 (GAG) antibody followed by donkey anti-mouse FITC-labeled secondary antibody. Hoechst 33258 was used for counterstaining of the nucleus (merged view). Bar: 20 μm (three independent experiments)Back to article page