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Fig. 3 | BMC Immunology

Fig. 3

From: Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determined by multiparametric single cell mass cytometry analysis

Fig. 3

Comparison of FC and MC detection of immunophenotyping markers in frozen primary tumor samples. a Ratio of percent positive cells detected by FC over MC for 19 common markers. Correlation between FC and MC was determined by Pearson Product Moment Correlation (PPMC). b Cell number and viability measurements. Viability and cell number as determined by FC analysis using an amine-reactive dye. Viability and number of acquired events as determined by MC analysis using 103Rh and 191/193Ir DNA intercalators. Cellular counts are expressed on a log10 scale. Data indicate that the viability and cell number differences acquired using FC and MC were major contributing factors in divergence of detected cellular frequencies in tumor samples

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BMC Immunology

ISSN: 1471-2172

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