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Fig. 3 | BMC Immunology

Fig. 3

From: An optimized IFN-γ ELISpot assay for the sensitive and standardized monitoring of CMV protein-reactive effector cells of cell-mediated immunity

Fig. 3

Effect of duration of antigen stimulation on IFN-γ ELISpot test results. SFC counts (means of four replicates) in IFN-γ ELISpot following stimulation of PBMC samples from three CMV-seropositive healthy donors (d120, 32-year-old male; d254, 62-year-old female; d270, 22-year-old female) with T-activated® IE-1 or pp65 for 17, 19 and 21 h. Unstimulated PBMC (neg.) were used as a negative control. Differences between stimulation durations were tested using the non-parametric two-sided One-way ANOVA Kruskal-Wallis test (*P < 0.05). P-values < 0.05 were considered statistically significant

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