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Fig. 5 | BMC Immunology

Fig. 5

From: An optimized IFN-γ ELISpot assay for the sensitive and standardized monitoring of CMV protein-reactive effector cells of cell-mediated immunity

Fig. 5

Comparison of IFN-γ ELISpot performance using different microtiter plates. ELISpot were performed using PBMC from three CMV-seropositive healthy donors (two preparations each, ELISpot in quadruplicate, ran twice) on various microtiter plate materials (MCE plate, PVDF plate, PVDF strips). Age (gender) of donors was 32 (male), 33 (male) and 51 (female). SFC values per 200,000 PBMC are shown as box plots for non-stimulated PBMC (neg.), and for T-activated® IE-1- and pp65-stimulated PBMC. Median values (horizontal black lines) are indicated in brackets. Differences between microtiter plate materials were tested using the non-parametric two-sided One-way ANOVA Kruskal-Wallis test (**P < 0.01; ***P < 0.001)

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BMC Immunology

ISSN: 1471-2172

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