Fig. 7
IFN-γ ELISpot assay linearity. a Working range of PBMC per ELISpot assay. Increasing number of PBMC from one CMV-seropositive healthy donor were seeded per well and ELISpot assays were performed as described, following stimulation with T-activated® IE-1 or pp65. Mean SFC values and standard deviation obtained for 60,000-200,000 PBMC per well are depicted. Scale of the Y-axes was adjusted for pp65 (left) and IE-1 (right) for a better data resolution. b Linearity between the number of CMV-reactive PBMC and enumerated SFC. The indicated numbers of PBMC from one CMV-seropositive healthy donor were mixed with PBMC from one CMV-seronegative donor (up to 200,000 total PBMC) and stimulated with T-activated® pp65 antigen according to the optimized protocol. Mean SFC values and standard deviation of quadruplicate measurements are shown for two donor pairs (d034 + d219, d204 + d067). In both panels, regression lines and corresponding coefficient of determination R2 were generated using the regression line tool of GraphPad Prism 5.04