Insight into SNPs and epitopes of E protein of newly emerged genotype-I isolates of JEV from Midnapur, West Bengal, India

Table 3 B cell specific antigenic peptide epitopes short listed from a large set of initial population based on their antigenic score, model energy, average ASA (Accessible surface area) and average conservation

Start Position	Linear Epitope	Score	Model Energy	Av. ASA	Av. H
325	ELTYSGSDGPCKIPIVSVAS	0.986	−23.71	22.8	0.01
372	VEMEPPFGDSYIVVGRGDKQ	0.977	−24.54	37.1	0.02
100	GWGKGCGLFGKGSIDTCAKF	0.956	−29.81	42.1	0.02
69	STVARCPTTGEAHNEKRADS	0.926	−12.95	59.3	0.01
395	HWHKAGSTLGKAFSTTLKGA	0.876	−23.38	97.2	0.02
121	CTSKAIGRMIQPENIKYEVG	0.854	−18.21	38.2	0.06
349	TPVGRLVTVNPFVATSSSNS	0.923	−20.55	39.9	0.04
48	IEASQLAEVRSYYYHASVTD	0.967	−32.65	31.4	0.06

NACCESS program [44] was used to generate residue accessibility data. Then average accessibility was computed using side chain relative accessibility values. Variability was calculated using full length E protein sequences (total 50) and positional Shannon entropy (H) [17, 18] was computed for each homologous position. The Av. Variability is the average of all residues value. Average is made up to 404 residues as the model has 404 residues like template

ISSN: 1471-2172