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Fig. 3 | BMC Immunology

Fig. 3

From: Forkhead-box transcription factor 1 affects the apoptosis of natural regulatory T cells by controlling Aven expression

Fig. 3

Proliferation and apoptosis after knockdown or over-expression of Foxo1 in Treg cells. a Treg cells growth were measured for 6 days using a MTT assay. Treg cells of every group (control, Foxo1-plasmid, Foxo1-RNAi and Foxo1-plasmid + CD127) except Foxo1-plasmid (no IL-7) was stimulated with anti-CD3 (0.01 μg/ml), anti-CD28 (1.0 μg/ml) and IL-7 (0.03 μg/ml) during culture, and anti-CD3 and anti-CD28 was only added in the Foxo1-plasmid (no IL-7) group. In addition, anti-CD127 (1:500) was added to the Foxo1-plasmid + CD127 group. These results are presented as the mean ± s.d. of the values obtained in three independent experiments. b Representative result of apoptosis in Treg cells based on the above groups 48 h after stimulation

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BMC Immunology

ISSN: 1471-2172

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