Fig. 4

Detection of Aven after knockdown and over-expression of Foxo1 in Treg cells. a Level of Foxo1 enrichment at the Aven gene promoters in Treg cells in which Foxo1 was knocked down and over-expressed. Expression of the (b) mRNA and (c) protein of Foxo1 and Aven in Treg cells 48 h after transfection with Foxo1 siRNA and over-expression. Expression of the (c) protein and (d) mRNA of Bcl2 in Treg cells 48 h after transfection with Foxo1 siRNA and over-expression. Cells was stimulated with anti-CD3 (0.01 μg/ml), anti-CD28 (1.0 μg/ml) and IL-7 (0.03 μg/ml) during culture. Data are presented as the mean + standard deviation (SD). *P < 0.01; **P < 0.005, ***P < 0.001