Fig. 1

Effect of lipopolysaccharide on histomorphology and ALT activity and in chicken liver. Following intraperitoneal LPS treatment in chickens at different time points, H&E staining was performed on liver serial tissue sections. Stellate macrophages (Kupffer cells) in perisinusoidal areas ①, diffuse infiltration of fat vacuoles indicating fatty infiltration ②, dilated central vein ③ and sinusoidal capillaries ④, reduction in size of a few hepatocytes ⑤, dissociated liver cells from each other in hepatic cords ⑥, dilated hepatic sinusoids along with fibrocytes proliferation in perisinusoidal areas ⑦, intracytoplasmic infiltration of variable size and shape fat vacuoles ⑧, dilated hepatic sinusoids ⑨, infiltration of oval shaped nucleated RBCs ⑩, cytoplasmic fat vacuoles have pushed hepatocyte nuclei at periphery ⑪, reduction in size of a few hepatocytes ⑫ and intense inflammatory cells infiltration around the portal area ⑬ (a). After LPS stimulation, alanine aminotransferase (ALT) activity was measured from liver tissues at 0 h, 2 h, 6 h, 12 h, 24 h, 36 h, 72 h and 120 h by ELISA technique (b). The letter C represents saline (control) group and L represents LPS group. The numbers represent the hours after stimulation. **P < 0.01