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Fig. 2 | BMC Immunology

Fig. 2

From: Lipopolysaccharide mediates immuno-pathological alterations in young chicken liver through TLR4 signaling

Fig. 2

Effect of LPS stimulation on hepatic cell proliferation in chicken liver. After intraperitoneal LPS injection in chicks at different time points, PCNA protein expression was assessed in liver tissue by immunohistochemistry using anti-PCNA antibody, PCNA positive product was mainly distributed around the portal and biliary epithelial cells and more concentrated expression was present on epithelial cell near portal area in saline group at 6 h, 12 h, 24 h, and 72 h as compared to LPS group (a). Quantification of PCNA expression from liver tissue images was accomplished by image-pro plus (IPP) computer software where IOD represents integrated optical density (b). The analysis of PCNA gene expression was performed by real-time quantitative RT-PCR and normalized by the expression of actin beta (ACTB) (c). The letter C represents saline (control) group and L represents LPS group. The numbers represent the hours after stimulation. *P < 0.05, **P < 0.01

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