Fig. 3

IL-7Rα blockade induces multiple co-inhibitory receptors in CD4⁺ and CD8⁺ T_E/M cells. a, b, c Prediabetic female NOD mice received anti-IL-7Rα (black circles; n = 10) or rat IgG (white circles; n = 10) antibodies as in Fig. 1a and blood was drawn and analyzed as in Figs. 1a and 2. Percentage of PD-1⁺CD44^high cells and MFI of extracellular Tim-3 and LAG-3 within the peripheral blood CD4⁺Foxp3⁻ (left) and CD8⁺ (right) T_E/M cell populations were determined by flow cytometry. All data are representative for two independent experiments. Each symbol represents one individual mouse. * = p ≤ 0.05, ** = p ≤ 0.005. d, e Frequency of Tim-3⁺ and LAG-3⁺ cells within the activated (CD44^high) CD4⁺Foxp3⁻ and CD8⁺ T_E/M cell populations in the PLN of mice treated twice per week for two consecutive weeks with anti-IL-7Rα (black circles) or rat IgG (white circles) antibodies. Data are pooled from two or three independent experiments (n = 3–8). * = p ≤ 0.05, ns = p > 0.05