Fig. 5

Co-inhibitory receptors reduce cytokine production in T cells from anti-IL-7Rα-treated mice. Female, prediabetic NOD mice (11 weeks old) were treated with anti-IL-7Rα or rat IgG antibodies twice a week for 2 weeks. At the end of treatment, spleen and PLN were harvested and pooled cells stimulated in vitro with anti-CD3 and anti-CD28 antibodies, in the absence (white circles) or presence of blocking antibodies against PD-L1, LAG-3 and Tim-3 (black diamonds). Supernatants were harvested after 18 h and IFN-γ (top panels) and IL-2 (bottom panels) levels determined by ELISA. Each symbol represents one individual mouse (n = 3). * = p ≤ 0.05, ** = p ≤ 0.005