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Fig. 5 | BMC Immunology

Fig. 5

From: Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies

Fig. 5

Detection of swine anti-C3 IgA, IgG and IgM in sera by iELISA. In the unvaccinated naturally PCV2-infected pig farm, 22 newborn piglets of TBP, were delivered from 11 sows during 4 seasons of 1 year. Blood samples were collected after parturition (after colostrum uptake), on the first day, the first month, the third month, and the 6th month of life, respectively. Serum samples from these pigs were used to detect anti-C3 specific antibodies by an iELISA. a Pig anti-C3 specific IgA were detected in these sera. b Pig anti-C3 specific IgG were detected in these sera. c Pig anti-C3 specific IgM were detected in these sera. d 16 PCV2-negative sera (4 each from 1-, 2-, 3-, and 6-month old pigs) were collected from the PCV2-negative herd in the primary specific pathogen free (SPF) pig facility. Serum samples from these pigs were used to detect anti-C3 specific antibodies (IgA, IgG, and IgM) by an iELISA. All samples were assayed twice. Each bar represents the mean ± SD of pig sera. Data shown were representative of two experiments. Significant p values are indicated as *p < 0.05. Statistical significance was calculated using paired Student’s t-test

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