Fig. 4
From: The immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection
ENO1 affects DC apoptosis. Flow Cytometry analysis was used to determine the rate of apoptosis in Chlamydia pulsed and nonpulsed WT and ENO1 knockdown DCs. DCs were stained with CD11c PE-CY7 stain and gated for further analysis. The DCs then were stained with Annexin V and 7-AAD. The Green fluorescence is FITC while the Red fluorescence is 7-AAD. Quadrant 4 (lower right) indicates cells undergoing apoptosis. The graph shows the average number of cells undergoing apoptosis and this has been depicted in their percentages. * Denotes statistical significance. This experiment was repeated 3 times