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Fig. 3 | BMC Immunology

Fig. 3

From: Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands

Fig. 3

Effect of a single concentration of pentraxin on macrophage markers. PBMC were cultured with either (a-c) 25 ng/ml M-CSF or (d-f) GM-CSF for 6 days and then SAP (10 μg/ml), CRP (10 μg/ml), or PTX3 (10 ng/ml) was added for an additional two days. Cells were then air-dried, fixed, and stained by ICC with antibodies against (a and d) CD163, b and e) CD169, c and f) CD206. Results shows the percent positive macrophages expressed as the mean ± SEM (n = 3–4 separate donors). *p < 0.05, **p < 0.01 (1-way ANOVA with Dunn’s test). g Representative images of PBMC cultured in the presence or absence of pentraxins and then stained for CD169. Bar is 0.1 mm. Insert shows a dendritic cell in PBMC cultured in GM-CSF

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