Fig. 4

Effect of M-CSF priming, pentraxin concentration, and pentraxin ligands on macrophage markers. PBMC were cultured in M-CSF for 6 days and then with increasing concentrations of (a, d, g) SAP, (b, e, h) CRP, or (c, f, i) PTX3, in the presence or absence of factor H (100 μg/ml), MBL (2 μg/ml), or C1q (30 μg/ml), for an additional two days. Cells were then air-dried, fixed, and stained by ICC with antibodies against (a-c) CD163, (d-f) CD169, and g-i) CD206. Results shows the percent positive macrophages expressed as the mean ±SEM (n = 4 CD163; n= 9 CD169; n= 4 CD206 separate donors)