Fig. 8

Effect of differentiation, pentraxin concentration, and pentraxin ligands on IL-10 production. PBMC were cultured for 6 days with increasing concentrations of (a) SAP, (b) CRP, or (c) PTX3, in the presence or absence of factor H (100 μg/ml), MBL (2 μg/ml), or C1q (30 μg/ml). Supernatants were then collected from the cells and assessed by ELISA for IL-10. Insert shows IL-10 production by SAP alone. Values are mean ± SEM (n = 3–4 separate donors). *p < 0.05 (1-way ANOVA with Dunn’s test)