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Table 1 Antibody list

From: Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands

Marker

Description of marker

Clone or Catalog number

Isotype

Source

CD163

hemoglobin-haptoglobin complex receptor

RM3/1

Mouse IgG1

BioLegend

CD169

Siglec-1 Sialoadhesin (Sn)

7-239

Mouse IgG1

BioLegend

CD172a/b

Signal regulatory protein α/β (SIRPα/β)

SE5A5

Mouse IgG1

BioLegend

CD200R

OX-2 receptor-cell surface receptor

OX-108

Mouse IgG1

BioLegend

CD206

Macrophage mannose receptor (MMR)

15-2

Mouse IgG1

BioLegend

CCR7 (CD197)

Receptor for chemokines CCL19/ELC and CCL21

3D12

Rat IgG2a

eBioscience

Dectin-1 (CLEC7A)

C-type lectin family

Clone 259931 MAB 1859

Mouse IgG2b

R&D Systems

Fibronectin

Matrix protein

EP5

Mouse IgG1

GeneTex

IFN-γ receptor (CD119)

Cytokine receptor

GIR-94

Mouse IgG2b

BD Bioscience

Interferon regulatory factor 4 (IRF4)

transcription factor

EP5699 (ab133590)

Rabbit monoclonal

Abcam

Interferon regulatory factor 5 (IRF5)

transcription factor

EPR6094 (ab124792)

Rabbit monoclonal

Abcam

MHC class II (DR)

major histocompatibility complex

L243

Mouse IgG2a

BioLegend

Raf1

serine/threonine-protein kinase

Y198 (ab32025)

Rabbit monoclonal

Abcam

RELM Beta (FIZZ 2)

resistin-like molecule beta Found in inflammatory zone 2

GTX88677

Goat polyclonal

GeneTex

TNFRSF14 (CD270)

cell surface receptor of the TNF-receptor superfamily

ab47677

Rabbit polyclonal

Abcam

sphingosine kinase 1 (SPHK1)

phosphorylates sphingosine to sphingosine-1-phosphate

GTX107509

Rabbit polyclonal

GeneTex