BMC Immunology

Table 1 Primer sequences of T helper type 17 (Th17)-related mouse cytokines

From: The imbalance of Th17/Treg cells is involved in the progression of nonalcoholic fatty liver disease in mice

Name		Primers (5’ ➝ 3’)	Product (bp)
β-actin	Sense	AGAGGGAAATCGTGCGTGAC	138 bp
β-actin	Antisense	CAATAGTGATGACCTGGCCGT	138 bp
IL-6	Sense	ACAACCACGGCCTTCCCTACTT	139 bp
IL-6	Antisense	CACGATTTCCCAGAGAACATGTG	139 bp
RORγt	Sense	GCCGCGGAGCAGACACACTT	176 bp
RORγt	Antisense	GGAGGCCCCCTGGACCTCTG	176 bp
Foxp3	Sense	CAGGAGAAAGCGGATACCAAATG	176 bp
Foxp3	Antisense	ATCTGTGAGGACTACCGAGCC	176 bp
STAT3	Sense	ACCTCCAGGACGACTTTGAT	203 bp
STAT3	Antisense	TGTCTTCTGCACGTACTCCA	203 bp

The housekeeping gene β-action was used as an internal control, and gene-specific mRNA expression was normalized against β-action expression. Relative quantification by the 2^-△△Ct method was realized by comparing to control groups

Back to article page

ISSN: 1471-2172

Contact us

General enquiries: journalsubmissions@springernature.com