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Fig. 4 | BMC Immunology

Fig. 4

From: Mercury alters endogenous phosphorylation profiles of SYK in murine B cells

Fig. 4

Quantitation of phosphorylation status within cluster regions of SYK. Phosphorylation of specific sites was quantified by MRM, and then standardized to a non-phosphorylated peptide (to control for SYK protein abundance) and normalized to untreated cells (to a value of 1.00). Results represent the average of 3 separate experiments, and each experiment was performed with biological triplicates. Error bars represent the Standard Error of the Mean (SEM). Phosphosites with localization probabilities ≥99% are in bold. (a) Monitoring of changes in phosphorylation status after exposure to 5, 25 or 50 μM Hg2+ for 10 min. (b) Monitoring of changes in phosphorylation status after exposure to either activating antibody (anti-IgM; 100 μg/106 cells for 2 min; red bars) or pervanadate (10 μM for 10 min; green bars). Statistical analyses were performed on the following clusters: S270 or T277 or S279 or S285; T339 or Y342 or S344 or Y346; Y519 or Y520; Y519 and Y520; and Y623 or Y624 or Y625. * = p ≤ 0.1; ** = p ≤ 0.05; *** = p ≤ 0.005

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