Table 1 Peptides and transition ions used for quantitative MRM analysis
From: Mercury alters endogenous phosphorylation profiles of SYK in murine B cells
Peptide | Phosphosite(s) | Parent m/z | Transition | Transition m/z | Collision Energy |
|---|---|---|---|---|---|
VLTVPC[+57]QK | Control | 472.765 | b2 | 213.16 | 14 |
VLTVPC[+57]QK | Control | 472.765 | y4 | 532.255 | 17 |
VLTVPC[+57]QK | Control | 472.765 | y6 | 732.371 | 14 |
IGAQM[+16]GHPGS[+80]PNAHPVTWSPGGIISR | S270 | 907.43 | y12 | 1269.695 | 37 |
IGAQM[+16]GHPGS[+80]PNAHPVTWSPGGIISR | S270 or T277 or S279 | 907.43 | y7 | 699.415 | 38 |
IGAQM[+16]GHPGS[+80]PNAHPVTWSPGGIISR | S270 or T277 or S279 or S285 | 907.43 | b7 | 711.324 | 36 |
IGAQM[+16]GHPGSPNAHPVTWS[+80]PGGIISR | T288 or S279 or S285 | 907.43 | y10-98 | 1055.563 | 41 |
IGAQM[+16]GHPGSPNAHPVTWS[+80]PGGIISR | T277 or S279 or S285 | 907.43 | y12-98 | 1251.684 | 37 |
EALPM[+16]DTEVY[+80]ESPYADPEEIRPK | T339 or Y342 or S344 or Y346 | 925.742 | b2 | 201.087 | 38 |
EALPM[+16]DTEVY[+80]ESPYADPEEIRPK | T339 or Y342 or S344 or Y346 | 925.742 | y4 | 513.351 | 38 |
EALPM[+16]DTEVY[+80]ESPYADPEEIRPK | T339 or Y342 or S344 or Y346 | 925.742 | y7 | 868.489 | 42 |
EALPM[+16]DTEVY[+80]ESPYADPEEIRPK | T339 or Y342 or S344 or Y346 | 925.742 | y20 | 1231.527 | 38 |
EALPM[+16]DTEVYESPY[+80]ADPEEIRPK | S344 or Y346 | 925.742 | y12 | 741.337 | 38 |
EALPM[+16]DTEVYESPY[+80]ADPEEIRPK | Y346 | 925.742 | y10 | 649.295 | 39 |
EALPM[+16]DTEVYESPY[+80]ADPEEIRPK | Y346 | 925.742 | y11 | 697.821 | 38 |
ALRADENY[+80]YK | Y519 | 661.792 | y2 | 310.176 | 28 |
ALRADENY[+80]YK | Y519 or Y520 | 661.792 | b5 | 527.294 | 23 |
ALRADENY[+80]YK | Y519 or Y520 | 661.792 | b6 | 656.336 | 23 |
ALRADENYY[+80]K | Y519 or Y520 | 661.792 | y3 | 277.107 | 24 |
ALRADENYY[+80]K | Y519 or Y520 | 661.792 | y3 | 553.206 | 20 |
ALRADENYY[+80.0]K | Y520 | 661.792 | y2 | 390.142 | 26 |
ALRADENY[+80.0]Y[+80.0]K | Y519Â +Â Y520 | 701.776 | b3 | 341.23 | 24 |
ALRADENY[+80.0]Y[+80.0]K | Y519Â +Â Y520 | 701.776 | b5 | 527.294 | 20 |
ALRADENY[+80.0]Y[+80.0]K | Y519Â +Â Y520 | 701.776 | b6 | 656.336 | 20 |
LRNYY[+80.0]YDVVN | Y623 or Y624 or Y625 | 699.808 | b7 | 1068.419 | 24 |
LRNYY[+80.0]YDVVN | Y623 or Y624 or Y625 | 699.808 | b8 | 1167.487 | 21 |
LRNYY[+80.0]YDVVN | Y623 or Y624 or Y625 | 699.808 | y2 | 232.129 | 24 |