Fig. 2

Blocking IL-10 signalling at the time of immunization does not increase the numbers of CD4 + Foxp3+ T cells. Group of 4 C57BL/6 mice were immunized with 50 μg of long E7 peptide/10 μg of MPLA, 50 μg of long E7 peptide/10 μg of MPLA/300 μg of anti-IL10R antibodies, 50 μg of long E7 peptide/10 μg of MPLA/300 μg of Normal Rat Serum or PBS twice respectively at 14 days apart subcutaneously, 1 weeks after final immunization; splenocytes and lymphocytes from draining lymph nodes were collected and single cells made; cells were stained for CD3, CD4 and intracellularly stained for Foxp3 as described in Methods. CD3+ cells were gated. Figure shows the numbers of CD4 + Foxp3+ cells in draining lymph nodes of different immunization groups in the draining lymph nodes (a) and spleen (b)