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Fig. 3 | BMC Immunology

Fig. 3

From: Blocking IL-10 signalling at the time of immunization does not increase unwanted side effects in mice

Fig. 3

Neutralizing IL-10 at the time of immunization does not cause inflammation in intestines. Group of 4 C57BL/6 mice were immunized with 50 μg of long E7 peptide/10 μg of MPLA, 50 μg of long E7 peptide/10 μg of MPLA/300 μg of anti-IL10R antibodies, 50 μg of long E7 peptide/10 μg of MPLA/300 μg of Normal Rat Serum or PBS twice at 14 days apart subcutaneously, 2 weeks or 3 months after final immunization. Mouse model of ulcerative colitis was induced by administering 5% DSS in the drinking water for 10 days. Samples of intestines were collected and examined for blood cells infiltration. a: Morphology of intestinal tract. b: Histology characterization of intestinal tract. Intestinal tract section were stained with hematoxylin/eosion (200 × magnification).c, d: Immunohistochemistry characterization of intestinal tract 2 weeks after final immunization. Intestinal tract section were incubated with anti- CD3 (c) or anti-Ly-6G mAb (d). e: Pathological score of intestinal tract. f: Expression of CD3 was calculated as integrated option density IOD /area (IOD/area) 2 weeks after final immunization. Results are from pooled two independent experiments (e, f)

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