Fig. 4

Neutralizing IL-10 at the time of immunization does not attract T cells and neutrophils to the intestines of immunized mice. Group of 4 C57BL/6 mice were immunized with 50 μg of long E7 peptide/10 μg of MPLA, 50 μg of long E7 peptide/10 μg of MPLA/300 μg of anti-IL10R antibodies, 50 μg of long E7 peptide/10 μg of MPLA/300 μg of Normal Rat Serum or PBS twice at 14 days apart subcutaneously. 1 weeks after final immunization; samples of intestines were collected and examined for blood cells infiltration as described in Methods. CD45+ cells were gated. a: Total CD45+ cells, b: CD3+ T cells, c: CD8+ T cells, d: CD4+ T cells, e: CD45 + CD11b + Ly6G+ cells