Fig. 2

iSN34 with CpG-B enhances IL-6 production. Supernatants from stimulated cells were collected and IL-6 protein levels were measured by ELISA. Mouse splenocytes were harvested 48 h later and intracellular IL-6 protein levels were determined by ELISA. All assays were carried out at least three independent times in triplicate. Similar results were obtained from at least three different mice. Values are presented as mean + SD of three independent experiments, each performed in triplicate (n = 9). Values with different letters (i.e., a, b, c, d, e, and f) were significantly different. ****p < 0.0001 vs. iSN34(0 μM). ND; not detectable