Fig. 7

CRP is negatively regulated by a phosphatidylinositol-3-kinase (PI3K) dependent pathway. a Mono Mac 6 cells were incubated in the absence (control), 1/10,000 dilution of DMSO, or 1 μM of the indicated compounds and then counted after 48 h. Values were normalized to the count of control cells. b-f Mono Mac 6 cells were incubated in the presence or absence of 75 μg/ml CRP and the indicated concentrations of b Syk inhibitor, c SP600125, d okadaic acid, e LY294002, e wortmannin, or g sodium vanadate. Cells were counted after 48 h and counts were normalized to the no CRP/ no inhibitor or + CRP with no inhibitor values. Data were fit to sigmoidal dose–response curves with a variable Hill coefficient. h Mono Mac 6 cells were incubated as in A in the presence of 75 μg/ml CRP. All values are mean ± SEM, n = 4 independent experiments. * p < 0.05, **p < 0.01 (1-way ANOVA, Dunnett’s test)