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Fig. 2 | BMC Immunology

Fig. 2

From: Mesenchymal stem cell conditioned medium alleviates oxidative stress injury induced by hydrogen peroxide via regulating miR143 and its target protein in hepatocytes

Fig. 2

MSC-CM treatment decreases apoptosis. H2O2: L02 incubated with 1 mM H2O2 for 3 h; H2O2 + MSC-CM: rescue group with addition of 20% MSC-CM for 24 h following H2O2 injury. a. MSC-CM protects L02 cells from apoptosis examined by Annexin V/PI double staining and flow cytometry. b The percentage of apoptotic L02 cells in the total cell population. c-d. MSC-CM protects L02 cells from MMP depolarization examined by JC-1 staining and flow cytometry. The lower right quadrant: MMP depolarization, a sign of early apoptosis (Mean ± SD. n = 10. * P < 0.05 and ** P < 0.01 vs. control group; # P < 0.05 vs. H2O2 group)

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BMC Immunology

ISSN: 1471-2172

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